A groundbreaking study conducted in the caves of Spain and Portugal reveals that ancient human DNA can persist for thousands of years on the surfaces of cave walls and rock art. This monumental discovery offers new insights into prehistoric humans and poses intriguing questions about whether Neanderthals engaged in cave painting.

“It’s the beginning of a new era,” says Genevieve von Petzinger, a researcher from the University of the Witwatersrand, Johannesburg, South Africa. “This allows us to potentially connect with the actual artist who created these remarkable works. It’s truly extraordinary.”

From 2022 to 2025, the First Art Project focused on dating the oldest cave paintings, collecting samples from 11 caves across Spain and Portugal featuring rock art. These paintings, believed to be among the earliest known forms, include graphic images such as triangles, dots, and hand stencils executed with red ocher paint. Researchers extracted small shavings of paint and calcite layers formed on cave walls via mineral precipitation.

Given that cave art is typically crafted using tools like fingers or by spitting paint, the researchers aimed to determine if the DNA of the artists had been preserved. While ancient human DNA has been known to exist in cave sediments, its presence on cave walls was previously undocumented.

This changed with the significant discovery of ancient human DNA in red spots that resemble semicolons within Portugal’s Escur Cave.

Nevertheless, this opens up exciting possibilities for identifying the creators of the artwork. “It’s as if the cave walls have transformed into the pages of an unfinished book, gradually filled with new discoveries,” states Hipolito Collado Giraldo, an Archaeologist from the government of Extremadura, Spain.

Another unexpected finding emerged when researchers sampled a control area of the cave wall devoid of artifacts, where they also discovered ancient human DNA. This genetic material likely originated from prehistoric visitors who touched the walls. “We were genuinely surprised,” says Corrado Giraldo. This suggests that even where cave paintings or archaeological items are absent, cave walls can unveil critical insights about ancient humans who frequented these sites.

Furthermore, the findings indicated that the DNA found on the walls of Escoral Cave came from direct contact with ancient humans, rather than sediment that had settled on the walls. In contrast to mixed animal DNA found in cave sediments, the genetic material on the Escoral walls solely consisted of human DNA.

The ancient DNA also provided intriguing insights into the individuals from whom it originated: three samples were primarily female and one was male. This genetic profile aligns closely with a group historically known as Western hunter-gatherers, dating from approximately 5,200 to 17,000 years ago.

Though the recovered DNA is insufficient for precise dating, it is known that Escoral Cave had been sealed off for the past 4,000 to 5,000 years, indicating the genetic material may be older.

This research marks only the beginning. Earlier this month, First Art researchers, including von Petzinger and Corrado Giraldo, conducted thorough sampling at various other caves in Spain, such as Nerja and Aldales, which are linked to Neanderthal art, although that connection remains contentious. “One question I am eager to explore is whether Neanderthals created art,” remarks Bossams-Mesa.

The potential of extracting ancient DNA from cave walls could revolutionize our understanding of ancient humans and their artistic expressions, asserts Francesco d’Errico from the University of Bordeaux, who was not associated with the study. “Did the artist identify as male, female, or perhaps even an animal? Were the drawings on the same panel crafted by the same individual? Will Neanderthal DNA eventually be discovered in the ancient paintings of the Iberian Peninsula, or does the hand stencil found in Indonesia contain Denisovan DNA? The possibilities are vast.”

However, ancient DNA was discovered in only one of the 24 rock art panels sampled, indicating that preservation may be more of an exception than a standard practice. “Currently, the success rate is quite low,” Bossams-Mesa observes. This may improve as researchers polish their methodologies for extracting minimal quantities of DNA from cave samples.

Corrado Giraldo expresses excitement about uncovering valuable information without the need for excavations, which can be destructive in nature. “Excavation inherently alters parts of the archaeological record,” he explains. “This groundbreaking discovery opens avenues for uncovering and reconstructing entirely new narratives without excavation—narratives that deepen our understanding of past peoples and societies.”