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First Discovery of Ancient Human DNA in Cave Paintings: Insights into Our Ancestry

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Ancient Rock Art Research

Samples Collected from Rock Art in Tiberin, Spain

Avamia Archeos Alberto Martinez Villa

A groundbreaking study conducted in the caves of Spain and Portugal reveals that ancient human DNA can persist for thousands of years on the surfaces of cave walls and rock art. This monumental discovery offers new insights into prehistoric humans and poses intriguing questions about whether Neanderthals engaged in cave painting.

“It’s the beginning of a new era,” says Genevieve von Petzinger, a researcher from the University of the Witwatersrand, Johannesburg, South Africa. “This allows us to potentially connect with the actual artist who created these remarkable works. It’s truly extraordinary.”

From 2022 to 2025, the First Art Project focused on dating the oldest cave paintings, collecting samples from 11 caves across Spain and Portugal featuring rock art. These paintings, believed to be among the earliest known forms, include graphic images such as triangles, dots, and hand stencils executed with red ocher paint. Researchers extracted small shavings of paint and calcite layers formed on cave walls via mineral precipitation.

Given that cave art is typically crafted using tools like fingers or by spitting paint, the researchers aimed to determine if the DNA of the artists had been preserved. While ancient human DNA has been known to exist in cave sediments, its presence on cave walls was previously undocumented.

This changed with the significant discovery of ancient human DNA in red spots that resemble semicolons within Portugal’s Escur Cave.

“It was a pleasant surprise,” notes Alba Bossams Mesa, from the Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany. “This is the first instance of ancient human DNA being identified on a cave wall. However, I cannot definitively conclude that this DNA belongs to the artist.” Bossams-Mesa adds, “It could be from someone who encountered the artwork at a later time, or perhaps even someone who merely sneezed.”

Nevertheless, this opens up exciting possibilities for identifying the creators of the artwork. “It’s as if the cave walls have transformed into the pages of an unfinished book, gradually filled with new discoveries,” states Hipolito Collado Giraldo, an Archaeologist from the government of Extremadura, Spain.

Pigment Samples Collected from Spain’s Altamira Cave Ceiling

Matthias Meyer

Another unexpected finding emerged when researchers sampled a control area of the cave wall devoid of artifacts, where they also discovered ancient human DNA. This genetic material likely originated from prehistoric visitors who touched the walls. “We were genuinely surprised,” says Corrado Giraldo. This suggests that even where cave paintings or archaeological items are absent, cave walls can unveil critical insights about ancient humans who frequented these sites.

Furthermore, the findings indicated that the DNA found on the walls of Escoral Cave came from direct contact with ancient humans, rather than sediment that had settled on the walls. In contrast to mixed animal DNA found in cave sediments, the genetic material on the Escoral walls solely consisted of human DNA.

The ancient DNA also provided intriguing insights into the individuals from whom it originated: three samples were primarily female and one was male. This genetic profile aligns closely with a group historically known as Western hunter-gatherers, dating from approximately 5,200 to 17,000 years ago.

Though the recovered DNA is insufficient for precise dating, it is known that Escoral Cave had been sealed off for the past 4,000 to 5,000 years, indicating the genetic material may be older.

This research marks only the beginning. Earlier this month, First Art researchers, including von Petzinger and Corrado Giraldo, conducted thorough sampling at various other caves in Spain, such as Nerja and Aldales, which are linked to Neanderthal art, although that connection remains contentious. “One question I am eager to explore is whether Neanderthals created art,” remarks Bossams-Mesa.

The potential of extracting ancient DNA from cave walls could revolutionize our understanding of ancient humans and their artistic expressions, asserts Francesco d’Errico from the University of Bordeaux, who was not associated with the study. “Did the artist identify as male, female, or perhaps even an animal? Were the drawings on the same panel crafted by the same individual? Will Neanderthal DNA eventually be discovered in the ancient paintings of the Iberian Peninsula, or does the hand stencil found in Indonesia contain Denisovan DNA? The possibilities are vast.”

However, ancient DNA was discovered in only one of the 24 rock art panels sampled, indicating that preservation may be more of an exception than a standard practice. “Currently, the success rate is quite low,” Bossams-Mesa observes. This may improve as researchers polish their methodologies for extracting minimal quantities of DNA from cave samples.

Corrado Giraldo expresses excitement about uncovering valuable information without the need for excavations, which can be destructive in nature. “Excavation inherently alters parts of the archaeological record,” he explains. “This groundbreaking discovery opens avenues for uncovering and reconstructing entirely new narratives without excavation—narratives that deepen our understanding of past peoples and societies.”

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Source: www.newscientist.com

Breakthrough Discovery: Tasmanian Tiger and Devil Paintings Found in Northern Australia

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The Tasmanian tiger (Thylacine) and the Tasmanian devil (Sarcophilus harrisii) are believed to have gone extinct on mainland Australia approximately 3,000 years ago. Currently, only 23 pieces of rock art featuring the Tasmanian devil and around 150 artworks depicting the Tasmanian tiger exist, mostly found at rock art sites in northern Australia. Recent discoveries in Arnhem Land, located in the Northern Territory of northwestern Australia, have unveiled 14 new images of previously undocumented Tasmanian tigers or quolls, along with two images of Tasmanian devils, with some dating back less than 1,000 years.

A naturalistic-style depiction of a Tasmanian tiger from Injarrak Hills, Northern Territory, Australia. Image credit: Craig Banggar.

The recently documented paintings of the Tasmanian tiger and devil are believed to date back approximately 15,000 years, rendered in various Aboriginal art styles using red and sometimes yellow ocher.

Additionally, artists utilized white pipe clay, which deteriorates over time and does not stain the rocks, making most white paintings less than 1,000 years old.

“The quoll was more widely dispersed across mainland Australia than the Tasmanian devil and held greater cultural significance, with only 25 recorded images of the Tasmanian devil compared to over 160 depictions of the quoll,” explained lead author Professor Paul Tassone from Griffith University.

“Those who painted more recent artworks may have actually observed live quolls, indicating some may have survived longer in Arnhem Land.”

“Alternatively, they could have drawn inspiration from older paintings.”

“Regardless, the quoll remains culturally significant today, with contemporary artists depicting Tasmanian tigers on bark, paper, and canvas, referred to as ‘Junkirk’.

“Retouched paintings in the region highlight the importance of these animals through generations,” added co-author Dr. Andrea Giarandoni, also from Griffith University.

“This petroglyph rock art provides crucial insights into historical human interactions with these animals.”

“These representations indicate that the quoll held a vital role in local culture and knowledge long before its extinction.”

Local oral traditions suggest that the Tasmanian tiger was symbolically linked to the Rainbow Serpent, often associated with water bodies.

“These creatures were integral to our ancestors’ lives,” remarked co-author Joey Ganjimira, a Jarama from western Arnhem Land.

“They frequently spoke of hunting alongside the possums.”

“Our study demonstrates that sugar gliders hold contemporary relevance in the region for both scientists and traditional communities,” said Professor Tassone.

“The sugar glider remains a living entity in western Arnhem Land, symbolizing ongoing cultural significance rather than merely being a relic of the past.”

This groundbreaking research is detailed in the following article: paper published in the latest issue of Archeology of Oceania.

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Paul SC Tasson et al. The Devil is in the Details: Photographic Records of Tasmanian Devils and Tasmanian Tigers from Aungbana and Injarak Hill, Northern Territory, Australia. Archeology of Oceania, published online March 30, 2026. doi: 10.1002/arco.70024

Source: www.sci.news

Oldest Evidence of Storytelling in Art Found in 51,200-Year-Old Indonesian Cave Paintings

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Archaeologists used a new technique called Laser Ablation U-series (LA-U-series) to re-date some of the oldest cave paintings in the Maros-Pangkep region of South Sulawesi and date stylistically similar motifs at other Maros-Pangkep sites. They found a minimum age of 50,200 years ago (± 2,200 years) for a hunting scene from the Leambur Sipong 4 cave site, first dated to a minimum of 43,900 years ago using a previous approach, meaning it is at least 4,040 years older than previously thought. They also assigned a minimum age of 53,500 years ago (± 2,300 years) to the newly described Leang Karampuang cave painting. Painted at least 51,200 years ago, the narrative work depicts human-like figures interacting with pigs and is now known as the world's oldest surviving example of figurative art and visual storytelling.

A 51,200-year-old mural from Leang Karampuan. Image courtesy of Griffith University.

Prehistoric rock art provides important insights into past human cultures, but is typically difficult to date in an accurate and reliable way.

Over the past few decades, solution-based U-series methods have been used to determine early dates for rock art in several regions, including Western Europe, island Southeast Asia, and Siberia.

In Spain, a handprint stencil was dated to at least 64,800 years ago by solution U-series analysis of the overlying calcite and attributed to Neanderthals.

Until now, the oldest evidence of representational art is a naturalistic painting of a warthog at Reang Tedonggae in Maros-Pangkep, Sulawesi, which was dated to at least 45,500 years ago using the solution U series.

“We have previously used uranium-series methods to date very ancient rock art in two areas of Indonesia, Sulawesi and Borneo, but our new laser ablation U-series (LA-U-series) technique is more accurate,” said Professor Maxim Aubert from Griffith University, lead author of the study.

“This will allow us to date the oldest calcium carbonate layers that formed in the murals and get a more accurate idea of when the murals were created. This will revolutionize the dating of murals.”

“The innovative technique we developed allows us to create a detailed 'map' of the calcium carbonate layers,” added study co-author Professor Renaud-Joannes Boyau from Southern Cross University.

“This feature makes it possible to precisely identify and avoid areas affected by natural diagenesis resulting from complex growth histories.”

“As a result, dating of rock art becomes more certain and reliable.”

The discovery that the Leang Karampuang paintings are at least 51,200 years old has important implications for understanding the origins of early art.

“Our results are very surprising. Apart from some controversial finds in Spain, none of the famous Ice Age rock arts in Europe are this old. This is the first time that Indonesian rock art has been dated to more than 50,000 years,” said the study's lead author Adi Agus Octaviana, a rock art expert at Jakarta's National Research and Innovation Agency and a doctoral student at Griffith University.

The scientists also used the LA-U series technique to re-date calcium carbonate deposits that lie above cave paintings at the Leang Bru Sipon 4 cave site.

The paintings, which consist of narrative “scenes” depicting figures interpreted as therianthropes (half-human, half-animal) hunting warthogs and dwarf buffalo, were estimated by the team to be at least 43,900 years old.

Using new techniques, the authors prove that the artwork dates to a minimum of about 48,000 years ago, making it 4,040 years older than present.

“The cave paintings at Leang Karampuang and Leang Bul Siphon 4 shed new light on a great period and the vital role of storytelling in art history,” said Professor Adam Blum of Griffith University, co-author of the study.

“It is remarkable that the oldest cave paintings found so far in Sulawesi consist of recognizable scenes – pictures of humans interacting with animals – and we can infer that the artists were trying to tell some kind of story.”

“This was a new discovery because the academic consensus on early figurative cave art was that it consisted of panels with single figures, no clearly defined scenes, and that pictorial representations that conveyed a narrative emerged much later in European art.”

This discovery suggests that narrative storytelling has been an important part of human artistic culture in Indonesia from very early times.

“Humans have probably been telling stories for much longer than 51,200 years, but because words don't fossilize we have to rely on indirect evidence like depicted scenes in artwork, and the Sulawesi artworks are the oldest such evidence known to archaeology so far,” Octaviana said.

of Investigation result Published in a journal Nature.

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AA Octaviana and othersl. The story of Indonesian cave paintings from 51,200 years ago. NaturePublished online July 3, 2024; doi: 10.1038/s41586-024-07541-7

Source: www.sci.news