Fungal sequences linked to lung issues have been identified from e-cigarette mouthpieces.

While vaping is often recommended as a means to avoid smoking, the long-term health impacts remain largely unclear.

Devices designed for vaping utilize battery-powered coils to heat liquids that typically contain nicotine, generating vapors for inhalation by the user. The primary health concerns center around the toxicity of the liquid’s chemical components; however, the presence of microorganisms transferred from the device to the user’s airways has not been thoroughly investigated.

For further insights, refer to Borna Mehrad and his team at the University of Florida, who studied 25 daily users of disposable vapes. Researchers isolated microorganisms from the mouthpiece of the devices and compared them with samples taken from the participants’ mouths.

Although the sample size was limited, researchers noted that more than half exhibited a “rich colonization” of fungal species distinct from those found in the participants’ mouths, with 80% of these species linked to potential illnesses in humans.

The predominant species identified was Cystobasidium minutum, which is associated with blood infections in immunocompromised individuals. To evaluate its impact on lung health, the research team exposed mice to C. minutum, mimicking the inhalation process associated with vaping.

“Our findings showed that the fungus most frequently found in vape samples induced characteristics of chronic bronchitis in mice,” explains Mehrad. This condition is characterized by airway inflammation and can result in flu-like symptoms.

Regarding the source of these fungi, some may be present in the environment or on our hands. However, Jason Smith indicated that while this has not been tested, it is possible that some microorganisms were already within the vaping liquid when purchased. “Residual material in the vape device can create a nutrient base for mold proliferation,” he adds.

Ian Musgrave from the University of Adelaide in Australia has noted that these fungi have also been detected in shisha (commonly referred to as hookah or waterpipe), which is known to contribute to lung diseases induced by microbes. “Notably, the microbiota documented in this study were predominantly composed of potentially pathogenic fungi that are uncommon in the oral microbiota,” he remarks.

Team member Katy Deitz expressed that the results were not surprising, considering that around one-third of participants who reported respiratory complaints, such as coughing, did not properly clean their vapes.

Musgrave advocates for regular cleaning of vaping devices but stresses that current evidence does not definitively indicate that these fungal species exist in sufficient quantities on vape mouthpieces to pose a health threat.