Frozen Squirrel Feces: A Unique Source for Preserving Ancient DNA from Hundreds of Species

Arctic ground squirrels forage for diverse food sources to stock their burrows.

Credit: Yukon Government

Recent analyses of DNA preserved in ancient frozen feces have unveiled a diverse ecosystem from 700,000 years ago, featuring species like woolly mammoths, bison, horses, and big cats.

The Arctic ground squirrel (Urositels parii) is a rodent measuring approximately 40 centimeters, inhabiting cold regions across North America and Siberia—historically linked by land bridges known as Beringia.

“Arctic ground squirrels hibernate for about eight months a year, so during their active four months, they venture out to forage and store resources in their burrows,” states Tyler Murchy from the Hakai Institute, Campbell River, Canada.

This foraging behavior results in their burrows often housing substantial amounts of feces and food, effectively making them “nature’s archivist,” according to Murchy. He and his team analyzed saprolites—preserved feces—frozen in permafrost from 13 Arctic ground squirrel burrows located in central Yukon, Canada.

Estimated to date back between 30,000 and 700,000 years, these burrows served as crucial sites for extracting DNA from various organisms present in the feces, which measured around 1 to 2 centimeters long.

The findings revealed a wealth of biodiversity, including microorganisms, over 200 different plant species, insects, other rodents, and large mammals such as woolly mammoths, horses, gray wolves, steppe bison, and lynx. An American cheetah or a cougar also inhabited this ecosystem. “All these organisms represent life from the Bering Ice Age,” Murchy remarks.

Contrary to popular belief, ground squirrels do not exclusively consume nuts and seeds. “They are actually quite omnivorous and have even been reported to scavenge on moose and lynx carcasses,” Murchy explains. Therefore, the discovery of such large animals in coprolites is not unexpected.

Ancient dung pellets from Arctic ground squirrels discovered in Yukon Territory, Canada

Credit: Duane Froese, University of Alberta

Utilizing the DNA extracted, Murchie and colleagues reconstructed the mitochondrial genomes of various species from different eras, including 12 ground squirrels—one lineage tracing back 700,000 years—three horses, two bison, and one hare. They also gathered enough DNA to reconstruct the genomes of six woolly mammoths, with further details pending publication.

“These remarkably preserved specimens showcase the ecological diversity of Yukon throughout history,” says Kelsey Witt of Clemson University, South Carolina.

Determining whether the DNA from any given species is present in coprolites because it was consumed by ground squirrels or because it existed in the environment can be challenging. However, the abundance of DNA within the samples and the scavenging behavior of the squirrels suggest they likely consumed mammoth meat, Witt concludes.

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The Rise of Dinosaurs Told through Fossilized Feces

A plant-eating sauropod dinosaur that evolved in a humid environment during the early Jurassic period.

Marcin Ambrozik

The contents of feces and vomit from 200 million years ago are helping to show how dinosaurs conquered the world at the beginning of the Jurassic period.

Whole insects embedded in various shapes and sizes of well-preserved plants, bones, fish parts, and even ancient animal feces show that dinosaurs varied in comparison to other groups of animals. This suggests that dinosaurs were able to survive in the ecosystem thanks to their wide-ranging diet. This led to their further growth and eventually led to the establishment of a “land dynasty.” Martin Kvarnström At Uppsala University, Sweden.

Fossil evidence shows that the first dinosaurs had prominent hip joints that placed their legs underneath their bodies like mammals, rather than sprawling sideways like lizards. More than 230 million years ago During the Triassic. For tens of millions of years, these early dinosaurs blended into a landscape filled with many other types of reptiles. But by about 200 million years ago, dinosaurs had essentially taken over the Earth, around the time most other reptiles disappeared during the end-Triassic extinction.

What led to this dominance remains somewhat of a mystery. Kvarnström and his colleagues suspected that important clues might be hidden in the bromalites (fossilized feces and vomit) of dinosaurs and other animals. So they Polish Geological Institute collected by a prior research group from eight locations in Poland between 1996 and 2017.

The research team estimated the age of each bromalite based on the layer of sediment in which it was found, and determined its size, which ranges from a few millimeters to a “fairly large fecal mass,” and the animals that may have produced it. A suitable shape was used. The researchers then 3D scanned the fossil to examine its contents. “We noticed that it was clogged with food debris,” Kvarnström says.

Coprolites, or fossilized feces, of herbivorous dinosaurs containing plant remains

Grzegorz Niedzwicki

Combining the known fossil record and past climate information, researchers determined that the emergence of dinosaurs occurred in several different stages. First, the omnivorous ancestors of early dinosaurs began to outnumber non-dinosaurs. They then evolved into the first carnivorous and plant-eating dinosaurs.

At that point, increased volcanic eruptions and movement of tectonic plates caused flooding and waterway development. The resulting humidity and related changes in climate likely led to a wider range of plants and the evolution of larger and more diverse herbivorous dinosaurs. On the other hand, animals other than dinosaurs (such as dicynodonts, which eat plants weighing up to 1 ton) Lisowisia, Their feces contained mainly coniferous debris and they were less able to adapt to changes in vegetation.

As herbivorous dinosaurs grew larger, so did their predators. By the beginning of the Jurassic period, about 30 million years after the first dinosaurs appeared, the transition to a dinosaur-dominated world was complete, Kvarnström says.

“This study shows how climate primarily affected dominant plants, which created opportunities for new herbivores at certain points in time,” he says. michael benton from the University of Bristol, UK, was not involved in the study.

Although it's difficult to be sure that the researchers matched the scat to the right animal, the discovery nonetheless suggests that the dinosaur species had already expanded significantly in South America before major climate change. This confirms earlier research, he says. “But it took the end-Triassic mass extinction for the final stage of the takeover to begin.”

for emma dunn The study, conducted at Germany's Friedrich-Alexander University, helps answer long-standing questions about the emergence of dinosaurs. “It's not every day that a fossil poop is published in such an influential journal,” said Dunn, who was not involved in the study. “This is obviously interesting, but it's also very useful for understanding prehistoric environments. So if you think of the evolution of early dinosaurs like a jigsaw puzzle with pieces missing, there are new It’s just a lot of pieces thrown in.”

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Source: www.newscientist.com