Unlocking Rich Chocolate Flavors: The Role of Cocoa Bean Microbiota

Chocolate is produced through the fermentation of cocoa beans sourced from cacao tree fruits.

Mimi Chu Leon

With the identification of fungi and bacteria that generate fruity caramel notes from cocoa beans, we were able to immediately experience a novel type of chocolate.

Typically, chocolate is crafted through the fermentation of cocoa beans extracted from the fruits of cocoa trees, followed by drying, roasting, and grinding them into a paste that is divided into cocoa butter and cocoa solids, mixed in varying ratios with different ingredients to create dark, milk, or white chocolate.

Throughout the fermentation process, surrounding microorganisms break down the cocoa fruit and create various compounds that enhance the chocolate’s flavor. This often results in a rich, earthy taste, according to David Salt from the University of Nottingham, UK. However, finely crafted chocolate can also exhibit fruity characteristics, which are frequently found in products from artisanal chocolate makers.

To investigate which microorganisms are responsible for these flavors, Salt and his team gathered samples of fermented beans from a cocoa farm in Colombia. By analyzing the genetic information within the samples, they discovered five types of bacteria and four fungi consistently present in batches of beans known for their exceptional flavor.

The researchers then introduced sterile cocoa beans to various microorganisms, fermented them with nine different microbial agents, and subsequently processed the beans into a liquid referred to as cocoa liquor. A panel of chocolate flavor experts assessed this liquor and noted the presence of fruity notes absent in samples made from beans without these microorganisms. “The infusion of these microorganisms imparted citrus, berry, floral, tropical, and caramel flavors,” says Salt.

The research indicates that incorporating these microorganisms into the fermentation blend may help cocoa growers enhance the flavor profile of their cocoa, leading to increased profits from their beans.

“We don’t necessarily need to introduce all nine microorganisms. There’s likely a practical approach to influence the microbiota favorably. For instance, we can confirm that specific fungi are naturally present outside of the cocoa pod,” he notes.

However, the group of microorganisms responsible for superior flavors may vary based on distinct cocoa farms, especially where environmental conditions differ. Further investigation is warranted, Salt advises.

Nonetheless, the study highlights that specific microorganisms can significantly amplify chocolate flavor, a finding that may also apply to varieties created from lab-grown cocoa, says Salt. Moreover, introducing a new microbial mix could even yield an entirely new type of chocolate.

topic:

  • Microbiology/
  • Food and drink

Source: www.newscientist.com

Study: Bean Consumption Enhances Metabolic and Inflammatory Indicators in Prediabetic Adults

A 12-week study involving 72 pre-diabetic adults revealed that the consumption of either chickpeas or black beans positively influences inflammation markers in diabetic patients. Additionally, chickpea intake helps in cholesterol regulation.

Incorporating one bean daily can yield significant benefits for both heart and metabolic health. Image credit: PDPICS.

“Pre-diabetic individuals often exhibit poor lipid metabolism and persistent low-grade inflammation, both of which can lead to diseases like heart disease and type 2 diabetes.”

“Our findings indicated that levels of tofu remained constant, yet they may aid in lowering cholesterol within pre-tofu individuals while also diminishing inflammation.”

While black beans and chickpeas are widely consumed, they are frequently neglected in extensive studies examining their effects on cholesterol and inflammation in those at risk for heart disease and diabetes.

This research forms part of a broader project investigating how the intake of black beans and chickpeas influences inflammation and insulin response mediated by intestinal microbiome activity.

“Our study highlights the advantages of bean consumption for pre-diabetic adults, but these legumes are excellent choices for everyone,” stated Smith.

“These insights can help shape dietary recommendations, clinical practices, and public health initiatives aimed at preventing heart disease and diabetes.”

To enhance the practical relevance of the research, the study was conducted with participants in their natural living environments.

Participants were randomly assigned to consume either 1 cup of black beans, chickpeas, or rice (the control group) over the span of 12 weeks.

Blood samples were collected at baseline, 6 weeks, and 12 weeks to monitor cholesterol levels, inflammation, blood glucose, and glucose tolerance tests were administered at both the beginning and conclusion of the study.

The group consuming chickpeas saw a significant drop in total cholesterol, from an average of 200.4 milligrams per deciliter at the start to 185.8 milligrams per deciliter after 12 weeks.

In the black bean group, the average level of the inflammatory cytokine interleukin-6, which is a marker for inflammation, decreased from 2.57 picograms per milliliter at baseline to 1.88 picograms per milliliter after 12 weeks.

No noteworthy changes were noted in markers of glucose metabolism.

“Switching to healthier alternatives, like canned, dried, or frozen beans, is an excellent starting point for those looking to increase their bean intake,” explained the scientist.

“However, it’s crucial to watch for extra ingredients like salt and sugar based on your selections.”

“There are numerous ways to include beans in your regular diet as a budget-friendly method to enhance your overall health and lower the risk of chronic ailments,” Smith added.

“You can blend them to thicken soups, use them as salad toppings, or combine them with other grains like rice or quinoa.”

The findings were reported in a presentation on June 3rd during the Nutrition 2025 annual meeting held by the American Nutrition Association.

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Morgan M. Smith et al. Effects of chronic intake of black beans and chickpeas on metabolism and inflammatory markers in prediabetic adults. Nutrition 2025 Summary #or18-01-25

Source: www.sci.news