Theobromine: A Dark Chocolate Compound That May Offer Anti-Aging Benefits

Researchers at King’s College London have found significant connections between theobromine, a widely-known plant compound from cocoa, and measures of epigenetic aging, indicating that theobromine may be associated with human aging.



third others. We illustrate that the documented beneficial relationship between health and aging and theobromine intake extends to the molecular epigenetic level in humans. Image credit: Sci.News.

“Coffee and cocoa are popular foods and are linked to lower rates of cardiovascular disease and mortality,” commented lead author Ramy Saad, Ph.D., along with colleagues.

“They contain several significant alkaloids, including theobromine, caffeine, theophylline, paraxanthine, and 7-methylxanthine.”

“Theobromine and 7-methylxanthine are partial metabolites of caffeine, yet both exist in much greater concentrations in cocoa as unprocessed primary metabolites.”

“Theobromine has long been associated with various health benefits and aging. For instance, studies in model organisms have confirmed a link between theobromine and extended lifespan.”

“Moreover, various human cohort observational studies have reported clear links between theobromine intake and multiple aspects of improved health.”

“Nonetheless, the exact impacts of theobromine on health and aging remain unclear, and the molecular pathways behind these effects are largely unknown.”

In the research, scientists analyzed the levels of theobromine in individuals’ blood against blood-based indicators of biological aging.

Across two European cohorts, which included 509 participants from TwinsUK and 1,160 from KORA, individuals with elevated levels of theobromine in their bloodstream exhibited a lower biological age compared to their chronological age.

“Our research discovered a correlation between key components in dark chocolate and prolonged youthfulness,” stated the study’s senior author, Professor Jordana Bell.

“While we’re not advocating for increased dark chocolate consumption, this study sheds light on how common foods might offer insights into healthier, longer living.”

The researchers also explored whether other metabolites found in cocoa and coffee reflected similar associations.

However, they concluded that the effect appears to be unique to theobromine.

Two different assessments were used to measure the biological age of participants.

Some researchers examined chemical alterations in DNA to estimate an individual’s aging rate.

Other scientists assessed the length of telomeres, the protective end caps of chromosomes, as telomere shortening is linked with aging and age-associated diseases.

“This is a fascinating finding, and the next crucial question is: What drives this association, and how can we further explore the interactions between dietary metabolites and the epigenome?” Dr. Saad remarked.

“This strategy could unveil significant discoveries about both common and rare diseases, as related to aging and beyond.”

“This study has uncovered another molecular mechanism through which natural compounds present in cocoa promote health,” noted study co-author Dr. Ricardo Costeira.

“Although further investigation is warranted, the findings highlight the importance of population-level analysis in the fields of aging and genetics.”

of findings Published in a journal on December 10th aging.

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Ramy Third others. Theobromine is associated with delayed epigenetic aging. aging published online on December 10, 2025. doi: 10.18632/aging.206344

Source: www.sci.news

Impact of Microbiota Composition, pH, and Temperature on Key Flavor Characteristics of Premium Chocolate

Cocoa (Theobroma cacao) bean fermentation is a natural process characterized by various interactions that influence the flavor profile of high-quality chocolate. By grasping these intricate interactions, one can effectively reproduce the sought-after flavor characteristics in a controlled environment. Research utilizing bean samples fermented at Columbia Farm has demonstrated that pH, temperature, and the composition of microbiota—encompassing both bacteria and fungi—significantly impact the essential flavor qualities of premium chocolate. This discovery lays the groundwork for developing fermentation starters aimed at consistently recreating the attributes of fine chocolate.

Gopaulchan et al. We have confirmed the previously suggested role of pH and temperature variations as reliable indicators of chocolate flavor properties. Image credit: Sci.News.

The creation of fermented products like chocolate relies on the metabolic activities of microbial communities.

These communities transform raw cocoa beans into essential precursors for chocolate production.

Once harvested, cocoa beans undergo several processing stages before becoming chocolate, but fermentation remains a spontaneous process.

“The distinctive flavor of chocolate is shaped by the fermentation of cocoa beans,” stated a representative from University of Nottingham.

“In contrast to the fermentation of wine, cheese, or dough, where specific microorganisms are added to enhance flavor, cocoa bean fermentation occurs naturally, and the microorganisms involved are not well understood.”

“The flavor profile of the beans is closely tied to the geographical location of the farm, resulting in variations in chocolate quality and taste.”

In this research, Dr. Castrillo and co-authors performed DNA sequence-based analyses on fermented cocoa beans from three separate farms in Colombia.

They discovered that a unique microbial community underpins the distinctive fermentation processes at Antioch farms, yielding a superior flavor, as validated by professional tasters.

By analyzing sequencing data, the authors identified the microbial interactions and metabolic pathways involved in fermentation.

This allowed for the design of microbial communities that could mimic the exquisite flavor of chocolate in laboratory settings. This was confirmed through evaluations by the same expert tasters and chocolate metabolite analyses.

Further studies could inform the development of industrial fermentation starters, eliminating the geographical limitations on chocolate flavor.

“The findings from this study enhance our understanding of how the composition of microbial communities during fermentation is a crucial factor in determining chocolate flavor properties,” stated the researchers.

“We have created a reliable methodology to design fermentation starters that facilitate the controlled domestication of the unpredictable microbial fermentations that occur on cocoa farms.”

“This paves the way for the evolution of the modern chocolate industry, akin to the beer and cheese sectors, based on regulated cocoa fermentation utilizing synthetic microbial starters that can consistently replicate the unique flavor characteristics of cocoa beans and chocolate.”

The team’s study was published in the journal Nature Microbiology this week.

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D. Gopaulchan et al. The defined microbial community recreates the attributes of finely flavored chocolate fermentation. Nat Microbiol Published online on August 18th, 2025. doi:10.1038/s41564-025-02077-6

Source: www.sci.news

Unlocking Rich Chocolate Flavors: The Role of Cocoa Bean Microbiota

Chocolate is produced through the fermentation of cocoa beans sourced from cacao tree fruits.

Mimi Chu Leon

With the identification of fungi and bacteria that generate fruity caramel notes from cocoa beans, we were able to immediately experience a novel type of chocolate.

Typically, chocolate is crafted through the fermentation of cocoa beans extracted from the fruits of cocoa trees, followed by drying, roasting, and grinding them into a paste that is divided into cocoa butter and cocoa solids, mixed in varying ratios with different ingredients to create dark, milk, or white chocolate.

Throughout the fermentation process, surrounding microorganisms break down the cocoa fruit and create various compounds that enhance the chocolate’s flavor. This often results in a rich, earthy taste, according to David Salt from the University of Nottingham, UK. However, finely crafted chocolate can also exhibit fruity characteristics, which are frequently found in products from artisanal chocolate makers.

To investigate which microorganisms are responsible for these flavors, Salt and his team gathered samples of fermented beans from a cocoa farm in Colombia. By analyzing the genetic information within the samples, they discovered five types of bacteria and four fungi consistently present in batches of beans known for their exceptional flavor.

The researchers then introduced sterile cocoa beans to various microorganisms, fermented them with nine different microbial agents, and subsequently processed the beans into a liquid referred to as cocoa liquor. A panel of chocolate flavor experts assessed this liquor and noted the presence of fruity notes absent in samples made from beans without these microorganisms. “The infusion of these microorganisms imparted citrus, berry, floral, tropical, and caramel flavors,” says Salt.

The research indicates that incorporating these microorganisms into the fermentation blend may help cocoa growers enhance the flavor profile of their cocoa, leading to increased profits from their beans.

“We don’t necessarily need to introduce all nine microorganisms. There’s likely a practical approach to influence the microbiota favorably. For instance, we can confirm that specific fungi are naturally present outside of the cocoa pod,” he notes.

However, the group of microorganisms responsible for superior flavors may vary based on distinct cocoa farms, especially where environmental conditions differ. Further investigation is warranted, Salt advises.

Nonetheless, the study highlights that specific microorganisms can significantly amplify chocolate flavor, a finding that may also apply to varieties created from lab-grown cocoa, says Salt. Moreover, introducing a new microbial mix could even yield an entirely new type of chocolate.

topic:

  • Microbiology/
  • Food and drink

Source: www.newscientist.com

Compounds in Chocolate and Tea Could Help Reduce Blood Pressure

A naturally occurring compound known as flavan-3-ol, present in cocoa, tea, apples, and grapes, has been shown to enhance blood pressure and vascular health, according to a recent meta-analysis conducted by researchers at the University of Surrey involving 145 prior studies.



Flavan-3-OL-rich foods and supplements, similar to medications, are regularly consumed, particularly by those with high blood pressure, to help lower blood pressure. Image credit: Lagou et al., doi: 10.1093/eurjpc/zwaf173.

“Flavan-3-ol found in cocoa, tea, and various fruits shows potential in enhancing blood pressure and vascular function, but is not yet utilized for cardiovascular prevention,” stated Professor Christian Heiss, the lead author of the study from the University of Surrey.

“Our meta-analysis evaluated how flavan-3-ol-rich interventions impact blood pressure and endothelial function.”

In the meta-analysis, the researchers examined data from 145 randomized controlled trials published between 1946 and 2024.

The findings indicated that regular intake of flavan-3-OLs could lead to reductions in blood pressure readings, particularly among individuals with elevated blood pressure or hypertension.

Some results showed that the average decrease in blood pressure was comparable to that produced by certain medications.

Additionally, flavan-3-ol was found to enhance endothelial function (the inner layer of blood vessels), which is crucial for overall cardiovascular health.

This improvement occurs independently of blood pressure changes, suggesting a broad positive impact on circulatory health.

“These results are promising for those seeking a simple way to manage blood pressure and support heart health through enjoyable dietary adjustments,” Professor Heiss added.

“Incorporating small amounts of commonly enjoyed foods such as tea, apples, dark chocolate, and cocoa powder into a balanced daily diet can provide beneficial flavan-3-ol levels.”

“While it should not replace prescribed medications or medical guidance, it can be a beneficial addition to a healthy lifestyle, particularly for individuals with high blood pressure, by incorporating flavan-3-ol-rich foods into their daily routine.”

“These encouraging findings warrant further research.”

A paper discussing the survey results has been published in the European Journal of Preventive Psychology.

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Vasiriki Ragou et al. Effect of flavan-3-OL on blood pressure and endothelial function in various populations: a systematic review and meta-analysis of randomized controlled trials. European Journal of Preventive Psychology Published online on March 24th, 2025. doi: 10.1093/eurjpc/zwaf173

Source: www.sci.news

Key Elements of Dark Chocolate Might Promote Healthy Aging

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                    <p class="ArticleImageCaption__Title">Dark chocolate is a notable source of theobromine, a beneficial chemical</p>
                    <p class="ArticleImageCaption__Credit">Studio-N/Shutterstock</p>
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    <p>While chocolate may be associated with anti-aging benefits, it’s wise to be cautious before indulging. The beneficial compounds are predominantly found in dark chocolate, and the overall health impact of chocolate consumption remains uncertain.</p>
    <p>"There are numerous aspects of dark chocolate, each with its pros and cons," says <a href="https://profiles.ucl.ac.uk/90100-ramy-saad/publications">Rumy Sars</a> from University College London.</p>

    <p>The substance in question, theobromine, is famously known to be toxic to dogs. However, that's just one aspect...</p>
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Source: www.newscientist.com

Delicious Chocolate Competes for Top Spot at Master Lab

Gabi Musat/500px/Getty Images

It is the rarest and most exclusive chocolate I have ever had. In fact, you can’t even buy it in the store. But that’s not that special. Just a few flat drops are slightly lighter in colour than most dark chocolate sealed in small plastic bags.

It smells like dark chocolate and tastes like that, but it doesn’t make it that bitter. Above all, for me, this is definitely true.

That’s important because what I’m eating wasn’t made using cocoa beans that were sourced from trees, like regular chocolate. Rather, it was grown in glass flasks by California cultureOne of several companies aiming to mass-produce chocolate in the bat using cell culture technology.

Cultivated chocolate may be even better than the types grown on trees, according to the company’s CEO Alan Perlstein, claiming that the level of chemicals, such as polyphenols, is high. There may be health benefitsthere is no contaminants such as heavy metals extracted from soil or pesticides, pesticides sprayed on crops, or taste currently on the market. “We are trying to create flavors that are almost unruly available through traditional chocolate production,” he says.

However, for many chocolate companies, the main attraction of getting raw wood from VATS instead of wood is its potentially unlimited supply. Climate change is hitting cacao farms violently, leading to shortages. Cocoa bean prices have quadrupled after staying relatively stable for decades.

So, can chocolate grown in VAT really compete with the diversity grown on trees at the price? And will consumers accept it?

It melts…

Source: www.newscientist.com

Study finds that consuming more dark chocolate, instead of milk, lowers risk of developing type 2 diabetes

A long-term US study found that consuming at least 5 servings of dark chocolate per week (1 serving equals a standard chocolate bar/pack or 1 oz) was associated with lower risk of type 2 diabetes compared to infrequent consumption. However, increased milk chocolate intake was associated with increased weight gain.

Consuming dark chocolate instead of milk chocolate may lower your risk of type 2 diabetes. Image credit: Sci.News.

The global prevalence of type 2 diabetes has increased significantly over the past few decades, with an estimated 463 million people affected worldwide in 2019 and projected to rise to 700 million by 2045. I am.

Type 2 diabetes is a multifactorial disease characterized by insulin resistance and impaired insulin secretion, which can lead to a number of serious complications, including cardiovascular disease, kidney failure, and vision loss.

A series of studies has highlighted the importance of lifestyle factors, such as a healthy diet, in the prevention and management of type 2 diabetes.

Higher total dietary flavonoid intake, as well as specific flavonoid subclasses, is associated with a lower risk of type 2 diabetes.

Randomized controlled trials have shown that these flavonoids exert antioxidant, anti-inflammatory, and vasodilatory effects that may benefit cardiometabolism and reduce the risk of type 2 diabetes, but the data are inconsistent. It wasn’t.

chocolate made from beans cacao tree (Theobroma cacao)one of the foods with the highest flavanol content and a popular snack around the world.

However, the association between chocolate intake and risk of type 2 diabetes remains controversial due to inconsistent results obtained in observational studies.

For new research, Liu Binkai Researchers at Harvard University's T.H. Chan School of Public Health combined data from three longitudinal U.S. observational studies of female nurses and male health care workers who had no history of diabetes, heart disease, or cancer at the time of recruitment. .

They investigated type 2 diabetes and total chocolate intake in 192,208 participants and 111,654 participants over an average 25-year monitoring period using food frequency questionnaires completed every 4 years. We analyzed the relationship between chocolate subtype (dark and milk) intake.

Because weight change strongly predicts type 2 diabetes risk, the researchers also used these food questionnaires to assess participants' total energy intake.

In the overall chocolate analysis, 18,862 people developed type 2 diabetes. After adjusting for personal, lifestyle, and dietary risk factors, the authors found that people who ate all types of chocolate at least five times a week were more likely to develop type 2 diabetes than those who ate little or no chocolate. We found that the incidence was significantly lower by 10%. .

In the chocolate subtype analysis, 4,771 people developed type 2 diabetes. After adjusting for the same risk factors, those who ate dark chocolate at least five times a week had a 21% significantly lower risk of type 2 diabetes, but there was no significant association with milk chocolate intake. was not found.

Researchers also found that each additional weekly intake of dark chocolate reduced the risk of type 2 diabetes by 3% (dose-response effect).

Increased milk intake was associated with long-term weight gain, but dark chocolate intake was not.

Dark chocolate has similar levels of energy and saturated fat as milk chocolate, but the high levels of flavanols found in dark chocolate reduce the risk of saturated fat and sugar for weight gain and other cardiometabolic diseases such as diabetes. may offset the effects of

“Increased consumption of dark chocolate, but not milk, was associated with a lower risk of type 2 diabetes,” the scientists said.

“Increased milk intake was associated with long-term weight gain, but dark chocolate intake was not.”

“Further randomized controlled trials are needed to replicate these findings and further investigate the mechanisms.”

of study What was posted this week BMJ.

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Liu Binkai others. 2024. Chocolate intake and risk of type 2 diabetes: A prospective cohort study. BMJ 387: e078386;doi: 10.1136/bmj-2023-078386

Source: www.sci.news

The covert chocolate garden designed to prevent the cocoa shortage

Give chocolate a fighting chance

Maciej Gorzelinski/EyeEm/Getty

READING will keep its secrets safe. Some might describe this town, 60 kilometers west of London, as nondescript. Exotic is certainly not the word. But hidden within a walled garden in a field on the south side of town is a special and unique destination. If it weren’t for what was going on here in the giant white tent, the chocolate would hit the stony road – nothing like marshmallows. This is the International Cocoa Quarantine Center. Find all your holiday reading here

Chocolate is the most popular sweet in the world. Globally, we eat 7 million tonnes of chocolate a year, and demand is on the rise as consumers in Asia also love the taste of chocolate. However, supply is never guaranteed. Most of the world’s commercial cocoa plants originate from just a few clones created in the 1940s, which have so far proven productive enough to meet demand. But this leads to a dangerous lack of genetic diversity, leaving cocoa vulnerable to the many pests and diseases that love it just like we do. Approximately 30 to 40 percent of crops are lost to disease each year, and there are concerns that climate change will worsen the problem. Efforts to breed new varieties of cocoa that are more productive, hardy and pest-resistant mean sending specimens around the world, which risks spreading disease and making matters worse. That is why, since 1985, the majority of cocoa samples transported to distant regions have made his two-year pit stop. “Today, Reading is the epicenter of the international cocoa movement,” says Andrew Daymond with some pride. He is a plant physiologist at the University of Reading and is in charge of cocoa quarantine. Once inside the tent, I am transported to the tropics. A wall of heat and humidity hit me, along with an impressive sight of hundreds of lush, green, two-meter-tall plants. Some had large orange or red pods hanging from their trunks. Daymond led me down a path of trees, stopping to snip a wrinkled yellow pod. Slice it open to reveal a white, slimy pulp with fatty brown seeds inside. The seeds are bitter and have only a slight chocolate taste. It is only after the seeds and pulp are fermented and the seeds are dried and roasted that the characteristic crunchy flavor begins to appear. “Why do we read?” I ask. It is a different world from the tropical forests of South America where cacao grows naturally. That’s exactly what’s important, Daymond says. Even if the pathogen were to escape, it would not survive long in Britain’s warm climate, and there are no crops in its native habitat to infect. In quarantine, Daymond and his team are keeping an eye out for fungal diseases that cause pods to rot, such as witch’s broom and the festive-sounding frostypod, both of which spread easily. In the 1990s, witch’s broom decimated cocoa production in the Brazilian state of Bahia after spores were introduced from the Amazon region, perhaps intentionally. Bahia’s production plummeted by 75%. So far, neither disease has reached West Africa. West Africa currently grows most of the world’s cocoa. They have various problems there. The insect-borne disease bud swollen virus can kill cocoa trees within a few years, and the bush beetle feeds on the pods, reducing yields by up to 40 percent. Yuri Cortes/AFP/Getty Images The cocoa samples arrive in Redding in the form of budwood (short sticks with many actively budding buds). Approximately 30 new varieties are introduced each year, including wild plants from rainforest expeditions. Upon arrival, samples are inspected for obvious signs of insect stowaway. The bud is then grafted onto a seedling to establish the mother plant. To check for any less obvious problems, buds from the mother plant are also grafted onto seedlings of an “indicator” plant, a type of cacao that exhibits more pronounced disease symptoms than other plants. If a virus or other disease is present in the incoming sample, symptoms will eventually develop. After two years, the research team is confident that the dormant virus will emerge and the plant will be deemed safe. Genetic tests being developed at the University of Reading could offer a way to speed up the isolation process, but Daymond says he is still not sure if these tests can detect all viruses. . Once the cacao trees are proven to be disease-free, cuttings are sent to researchers around the world. One of them is Wilbert Phillips Mora, a cocoa disease expert and head of the breeding program at Costa Rica’s Center for Advanced Education in Tropical Agriculture (CATIE). For decades, he has painstakingly mixed promising strains to create hybrids that are screened for disease resistance. “We are refreshing the cocoa blood,” says Phillips Mora. His CATIE R6, one of the new varieties he developed, not only shows remarkable resistance to frosty pods, but also significantly increases productivity. The chocolate decorating the cake was honored in 2009 at the International Cocoa Awards for its taste and aroma. quite a pile of beans New varieties such as CATIE R6 are sent to researchers in other countries to cross with indigenous crops and deploy to farmers. Many plantations, particularly in West Africa, are reaching the end of their productive life. This new blood is desperately needed.. The quarantine greenhouse is the size of four tennis courts, and most of it is already filled with all-cleared plants (400 varieties). Plants still in quarantine will be kept separately. Has anyone made chocolate from the Redding crop, preferably ask Daymond. “That’s not something we tried,” he says. “You need heaps of beans to properly ferment cocoa beans, and we don’t have a lot of pods available here.” Regardless, I decided to give it a try when I got home. I placed the contents of the single cacao pod Daymond gave me in the most tropical environment I could find, next to a hot water tank. First, the pulp must be fermented into an alcoholic liquid to break down the astringent compounds contained in the seeds. The seeds are then dried and roasted in the oven. easy. Or not, after all. A few days later, I noticed that there were a few moldy black beans in the bag, and it had a not-so-appetizing aroma. Leave the chocolate making to the experts and enjoy the exotic flavors of Redding in every bite. This article was printed under the heading “Away from Chalk”.

Source: www.newscientist.com