Unlocking Rich Chocolate Flavors: The Role of Cocoa Bean Microbiota

Chocolate is produced through the fermentation of cocoa beans sourced from cacao tree fruits.

Mimi Chu Leon

With the identification of fungi and bacteria that generate fruity caramel notes from cocoa beans, we were able to immediately experience a novel type of chocolate.

Typically, chocolate is crafted through the fermentation of cocoa beans extracted from the fruits of cocoa trees, followed by drying, roasting, and grinding them into a paste that is divided into cocoa butter and cocoa solids, mixed in varying ratios with different ingredients to create dark, milk, or white chocolate.

Throughout the fermentation process, surrounding microorganisms break down the cocoa fruit and create various compounds that enhance the chocolate’s flavor. This often results in a rich, earthy taste, according to David Salt from the University of Nottingham, UK. However, finely crafted chocolate can also exhibit fruity characteristics, which are frequently found in products from artisanal chocolate makers.

To investigate which microorganisms are responsible for these flavors, Salt and his team gathered samples of fermented beans from a cocoa farm in Colombia. By analyzing the genetic information within the samples, they discovered five types of bacteria and four fungi consistently present in batches of beans known for their exceptional flavor.

The researchers then introduced sterile cocoa beans to various microorganisms, fermented them with nine different microbial agents, and subsequently processed the beans into a liquid referred to as cocoa liquor. A panel of chocolate flavor experts assessed this liquor and noted the presence of fruity notes absent in samples made from beans without these microorganisms. “The infusion of these microorganisms imparted citrus, berry, floral, tropical, and caramel flavors,” says Salt.

The research indicates that incorporating these microorganisms into the fermentation blend may help cocoa growers enhance the flavor profile of their cocoa, leading to increased profits from their beans.

“We don’t necessarily need to introduce all nine microorganisms. There’s likely a practical approach to influence the microbiota favorably. For instance, we can confirm that specific fungi are naturally present outside of the cocoa pod,” he notes.

However, the group of microorganisms responsible for superior flavors may vary based on distinct cocoa farms, especially where environmental conditions differ. Further investigation is warranted, Salt advises.

Nonetheless, the study highlights that specific microorganisms can significantly amplify chocolate flavor, a finding that may also apply to varieties created from lab-grown cocoa, says Salt. Moreover, introducing a new microbial mix could even yield an entirely new type of chocolate.

topic:

  • Microbiology/
  • Food and drink

Source: www.newscientist.com

Researchers warn that the rapid spread of a virus poses a threat to the health of cocoa trees

Cocoa swollen bud virus disease It is one of the most economically damaging cocoa tree diseases, accounting for almost 15-50% of crop losses in Ghana. This virus is transmitted when several species of mealybugs feed on cocoa plants.

A cacao tree with cacao fruit attached directly to the trunk or branches.Image credit: Gamera other., doi: 10.1094/PDIS-10-22-2412-FE.

Cocoa swollen shoot virus disease (CSSVD) first observed Infected by farmers in the eastern region of Ghana in 1936, the nature of the virus was identified in 1939.

CSSVD is considered the most economically damaging cocoa virus disease and can cause yield losses of 15-50% when severe strains are involved in infection.

Cocoa swollen shoot virus (CSSV) is classified as a member of the plant-infecting pararetroviruses of the genus Cocoa swollen shoot virus. badnavirus It contains nonenveloped rod-like particles that encapsulate a circular double-stranded DNA genome.

Previously, isolates and strains were grouped according to severity of symptom onset and geographic origin.

CSSVD is currently known to be caused by the following causes: complex of badnavirus seed Based on molecular structure.

CSSV affects all parts of the cocoa plant. Symptoms seen on leaves include red vein stripes on immature “flush” leaves, green-green vein spots or stripes that can occur in angular spots, removal of green-green veins, and various shapes Includes mosaic symptoms.

The virus causes swelling of the stem (nodes, internodes, tips) and roots.

In some strains, the infected pods change shape, becoming rounder and smaller and with a smoother surface.

“This virus is a real threat to the world's chocolate supply,” said Professor Benito Chen Charpentier, a researcher at the University of Texas at Arlington.

“Pesticides are ineffective against mealybugs, so farmers try to prevent the spread of the disease by cutting down infected trees or breeding resistant trees. However, Ghana has lost more than 254 million cocoa trees in recent years.

Farmers can combat mealybugs by vaccinating trees with CSSV. But vaccines are expensive, especially for low-wage farmers, and vaccinated trees produce less cocoa, making the damage caused by the virus even worse.

Electron micrograph of swollen shoot virus particles in purified cocoa. Scale bar – 200 nm.Image credit: Gamera other., doi: 10.1094/PDIS-10-22-2412-FE.

Professor Chen Charpentier and colleagues have developed a new strategy. Use mathematical data to determine how far apart farmers can plant vaccinated trees to prevent mealybugs from jumping from one tree to another and spreading the virus That's what it is.

“Mealybugs have several ways of getting around, including moving from canopy to canopy, being carried by ants, and being blown by the wind,” Professor Chen Charpentier said.

“What we needed to do was create a model for cocoa farmers to vaccinate unvaccinated trees to prevent the spread of the virus while making costs more manageable for smallholders. The goal was to be able to know how far away a tree could be safely planted.”

By experimenting with mathematical patterning techniques, the authors create two different types of models that allow farmers to create a protective layer of vaccinated cocoa trees around unvaccinated trees. Did.

“Although still experimental, these models are exciting because they can help farmers protect their crops while achieving better yields,” said Professor Chen Charpentier.

“It’s good for farmers’ bottom lines and it’s good for the world’s chocolate addiction.”

a paper The findings were published online in the journal PLoS ONE.

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FB Agusto other. 2024. Cocoa Sustainability: The Case of Cocoa Swollen Bud Virus Co-infection. PLoS ONE 19 (3): e0294579; doi: 10.1371/journal.pone.0294579

Source: www.sci.news

The covert chocolate garden designed to prevent the cocoa shortage

Give chocolate a fighting chance

Maciej Gorzelinski/EyeEm/Getty

READING will keep its secrets safe. Some might describe this town, 60 kilometers west of London, as nondescript. Exotic is certainly not the word. But hidden within a walled garden in a field on the south side of town is a special and unique destination. If it weren’t for what was going on here in the giant white tent, the chocolate would hit the stony road – nothing like marshmallows. This is the International Cocoa Quarantine Center. Find all your holiday reading here

Chocolate is the most popular sweet in the world. Globally, we eat 7 million tonnes of chocolate a year, and demand is on the rise as consumers in Asia also love the taste of chocolate. However, supply is never guaranteed. Most of the world’s commercial cocoa plants originate from just a few clones created in the 1940s, which have so far proven productive enough to meet demand. But this leads to a dangerous lack of genetic diversity, leaving cocoa vulnerable to the many pests and diseases that love it just like we do. Approximately 30 to 40 percent of crops are lost to disease each year, and there are concerns that climate change will worsen the problem. Efforts to breed new varieties of cocoa that are more productive, hardy and pest-resistant mean sending specimens around the world, which risks spreading disease and making matters worse. That is why, since 1985, the majority of cocoa samples transported to distant regions have made his two-year pit stop. “Today, Reading is the epicenter of the international cocoa movement,” says Andrew Daymond with some pride. He is a plant physiologist at the University of Reading and is in charge of cocoa quarantine. Once inside the tent, I am transported to the tropics. A wall of heat and humidity hit me, along with an impressive sight of hundreds of lush, green, two-meter-tall plants. Some had large orange or red pods hanging from their trunks. Daymond led me down a path of trees, stopping to snip a wrinkled yellow pod. Slice it open to reveal a white, slimy pulp with fatty brown seeds inside. The seeds are bitter and have only a slight chocolate taste. It is only after the seeds and pulp are fermented and the seeds are dried and roasted that the characteristic crunchy flavor begins to appear. “Why do we read?” I ask. It is a different world from the tropical forests of South America where cacao grows naturally. That’s exactly what’s important, Daymond says. Even if the pathogen were to escape, it would not survive long in Britain’s warm climate, and there are no crops in its native habitat to infect. In quarantine, Daymond and his team are keeping an eye out for fungal diseases that cause pods to rot, such as witch’s broom and the festive-sounding frostypod, both of which spread easily. In the 1990s, witch’s broom decimated cocoa production in the Brazilian state of Bahia after spores were introduced from the Amazon region, perhaps intentionally. Bahia’s production plummeted by 75%. So far, neither disease has reached West Africa. West Africa currently grows most of the world’s cocoa. They have various problems there. The insect-borne disease bud swollen virus can kill cocoa trees within a few years, and the bush beetle feeds on the pods, reducing yields by up to 40 percent. Yuri Cortes/AFP/Getty Images The cocoa samples arrive in Redding in the form of budwood (short sticks with many actively budding buds). Approximately 30 new varieties are introduced each year, including wild plants from rainforest expeditions. Upon arrival, samples are inspected for obvious signs of insect stowaway. The bud is then grafted onto a seedling to establish the mother plant. To check for any less obvious problems, buds from the mother plant are also grafted onto seedlings of an “indicator” plant, a type of cacao that exhibits more pronounced disease symptoms than other plants. If a virus or other disease is present in the incoming sample, symptoms will eventually develop. After two years, the research team is confident that the dormant virus will emerge and the plant will be deemed safe. Genetic tests being developed at the University of Reading could offer a way to speed up the isolation process, but Daymond says he is still not sure if these tests can detect all viruses. . Once the cacao trees are proven to be disease-free, cuttings are sent to researchers around the world. One of them is Wilbert Phillips Mora, a cocoa disease expert and head of the breeding program at Costa Rica’s Center for Advanced Education in Tropical Agriculture (CATIE). For decades, he has painstakingly mixed promising strains to create hybrids that are screened for disease resistance. “We are refreshing the cocoa blood,” says Phillips Mora. His CATIE R6, one of the new varieties he developed, not only shows remarkable resistance to frosty pods, but also significantly increases productivity. The chocolate decorating the cake was honored in 2009 at the International Cocoa Awards for its taste and aroma. quite a pile of beans New varieties such as CATIE R6 are sent to researchers in other countries to cross with indigenous crops and deploy to farmers. Many plantations, particularly in West Africa, are reaching the end of their productive life. This new blood is desperately needed.. The quarantine greenhouse is the size of four tennis courts, and most of it is already filled with all-cleared plants (400 varieties). Plants still in quarantine will be kept separately. Has anyone made chocolate from the Redding crop, preferably ask Daymond. “That’s not something we tried,” he says. “You need heaps of beans to properly ferment cocoa beans, and we don’t have a lot of pods available here.” Regardless, I decided to give it a try when I got home. I placed the contents of the single cacao pod Daymond gave me in the most tropical environment I could find, next to a hot water tank. First, the pulp must be fermented into an alcoholic liquid to break down the astringent compounds contained in the seeds. The seeds are then dried and roasted in the oven. easy. Or not, after all. A few days later, I noticed that there were a few moldy black beans in the bag, and it had a not-so-appetizing aroma. Leave the chocolate making to the experts and enjoy the exotic flavors of Redding in every bite. This article was printed under the heading “Away from Chalk”.

Source: www.newscientist.com