Paleontologists Uncover Earliest Evidence of Ancient Honey Bees Nesting in Vertebrate Fossil

Bees exhibit an extraordinary range of species and behavior, from solitary types that nest in burrows to social variants that construct intricate nests. This nesting diversity is partially captured in the fossil record, with trace fossils dating from the Cretaceous to the Holocene. In a recent study, Field Museum paleontologist Lázaro Vignola López and his colleagues unveil novel nesting behaviors based on trace fossils unearthed from Late Quaternary cave deposits on the Caribbean island of Hispaniola. Osnidam Almontei was discovered within the cavity of a vertebrate corpse.



A reenactment of the life of bees with evidence of them building their nests in caves and using the bone cavities as housing chambers for several egg-laying cells. Image credit: Jorge Mario Macho.

“The initial descent into the cave isn’t very deep. You secure a rope to the side and rappel down,” Dr. Vignola López explained.

“Entering at night, you can spot the eyes of tarantulas residing there. As you navigate through the 10-meter-long underground tunnel, fossils start to emerge.”

Fossil layers were separated by carbonate deposits formed during rainy periods in antiquity.

Although rodents made up most of the fossils, remains of sloths, birds, and reptiles—over 50 species in total—were also found. Together, these fossils narrate a compelling story.

“We hypothesize this cave served as a home for owls over generations, potentially for hundreds or even thousands of years,” Dr. Vignola López remarked.

“The owl would venture out to hunt and return to the cave to regurgitate pellets.”

“We’ve collected fossils of the prey the owl consumed, its own remains, and even bones of turtles and crocodiles that may have inadvertently fallen into the cave.”

Dr. Vignola López and his colleagues observed that the sediments in empty tooth sockets of mammalian jaws didn’t accumulate randomly.

“The surface was notably smooth and slightly concave, which is unusual for sediment burial. I noticed this pattern across multiple specimens and thought to myself, ‘There’s something peculiar here.’ It reminded me of a hornet’s nest,” he noted.

Many well-known nests constructed by bees and wasps belong to social species that coexist in large colonies, raising their young together—like the bees in honeycombs or paper wasp nests.

“However, the majority of bee species are solitary. They deposit eggs in small cavities and leave pollen for their larvae’s nourishment,” Dr. Vignola López continued.

“Some bee species create nests in tree hollows, in the ground, or utilize vacant spaces. Certain species in Europe and Africa even nest within discarded snail shells.”

To investigate the possible insect nests within the cave fossils, the researchers conducted CT scans and X-rays of the bones, capturing 3D images of the compacted soil in the tooth sockets without damaging the fossils.

The shape and composition of the deposits bore similarities to mud nests created by some contemporary bee species. Some of these nests contained ancient pollen grains that mother bees had sealed for their larvae.

The researchers propose that bees combined saliva and soil to construct small nests for their eggs, smaller than the eraser on a pencil.

Nesting within larger animal bones provided protection for bee eggs from potential predators such as wasps.

While the bees themselves were not preserved, the unique characteristics of the nests allowed for a taxonomic classification.

They named the nests Osnidam Almontei, in honor of the scientist Juan Almonte Milan, who first discovered this cave.

“Since no bee remains were found, they may have belonged to a currently existing species. Our knowledge on the ecology of various bee species in these islands is limited,” Dr. Vignola López stated.

Scientists speculate that this behavior arises from a combination of factors. With little soil covering the limestone terrain in this region, bees may have opted for caves as their nesting sites, rather than digging into the ground like many other species.

Additionally, this cave had been home to generations of owls, with numerous owl pellets accumulating over the years, providing the bees with ample use of the bones the owls left behind.

“This finding illustrates the peculiarities of bees. They can be surprising, and it emphasizes the importance of meticulous examination when studying fossils,” Dr. Vignola López remarked.

of paper published today in Proceedings of the Royal Society B Biological Sciences.

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Lazaro W. Vignola-Lopez and others. 2025. Fossil traces in mammal remains reveal a new nesting behavior for bees. R Soc Open Science 12(12):251748; doi: 10.1098/rsos.251748

Source: www.sci.news

Archaeologists Discover 2,500-Year-Old Honey in Bronze Jars Unearthed in Italy

Archaeologists have reexamined the 2,500-year-old remnants discovered in bronze containers at an underground shrine in Paestum, Italy, previously categorized as a mixture of wax, fat, and resin. Utilizing a multi-species approach, the researchers identified lipids, sugar degradation products, hexose sugars, and key royal jelly proteins.

Paestum Honey: (a) An underground shrine in Paestum, Italy. (b) One of the Hydrias showcased in 2019 alongside a Perspex box containing Ashmolean Museum residues. (c) A graphic representation of the arrangement of bronze artifacts within the shrine. (d) Samples extracted from the core of the residue. Image credit: Da Costa Carvalho et al. , doi: 10.1021/jacs.5c04888.

Honey played a crucial role in ancient societies.

Historical texts and depictions indicate that honey was utilized as an early sweetener in medicinal recipes, rituals, and cosmetics.

Within ancient Greek and Roman traditions, bees and honey were of significant religious and symbolic value.

Honey is thought to confer wisdom, with myths stating that Zeus was given honey in his infancy.

Discovering honey in archaeological residues offers direct chemical evidence of the collection, exploitation, and processing of bee products, illuminating early agricultural and subsistence strategies in various regions globally.

In 1954, excavations at an ancient Greek site in Pestum, southern Italy, dating back to around 520 BC, unveiled an underground shrine dedicated to an unknown deity. This shrine housed a bronze bottle and two amphoras encircling an empty iron bed.

The container was found to hold a paste-like residue with a distinct waxy aroma.

Archaeologists noted that the residue was initially a liquid or semi-liquid. Traces were discovered on the exterior of a vessel that had originally been sealed with a cork disk.

The excavation reports underscored the shrine’s sacredness, with the empty bed’s inaccessibility suggesting the presence of the deity.

Additionally, archaeologists identified the original contents of the bronze bottle as honey, a revered symbol believed to have originated from honeycomb, though only traces of beeswax persisted as a prominent element.

Subsequent laboratory analyses of samples with varying residues ruled out honey from the composition.

In 2019, when the residues from Paestum reached the Ashmolean Museum for display during the final evening of the Pompeii exhibition, it presented a fresh opportunity to reassess the biomolecular composition, leveraging recent advances in mass spectrometry technology.

Researchers from Oxford University, including Luciana da Costa Carvalho and James McCulla, analyzed the residue samples, determining their molecular composition through several modern analytical methods.

The analysis revealed that the ancient residues bore nearly identical chemical signatures to modern beeswax and honey, with elevated acidity levels indicative of changes due to prolonged storage.

The chemical profile of the residue appears to be more complex than that of pyrolyzed beeswax, hinting at the existence of honey or other substances.

Residues in contact with the bronze jar showed a mixture of sugars that had reacted with copper.

Hexose sugars, frequently identified in honey, were present in higher concentrations in the ancient residues compared to modern beeswax.

Proteins linked to Royal Jelly, known to be secreted by western honeybees, were also identified in the residues.

These findings suggest that the ancient material may indeed be remnants of ancient honey.

However, researchers have not entirely ruled out the presence of other bee products.

“The ancient residues are not simply traces of what people consumed and offered to the gods; they represent intricate chemical ecosystems,” remarked Dr. Da Costa Carvalho.

“Investigating these substances will unveil how they have evolved over time, paving the way for future studies on ancient microbial activities and their potential applications.”

A paper detailing the study’s findings was published today in the Journal of the American Chemical Society.

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Luciana da Costa Carvalho et al. Immortal symbol: evidence of honey in bronze jars from 530 to 510 BC. J. Am. Chemistry. Soc, published online on July 30th, 2025. doi:10.1021/jacs.5c04888

Source: www.sci.news

Ancient Pot Near Pompeii Holds Honey from 2,500 Years Ago

Ancient Greek bronze jars displayed at the Ashmolean Museum in Oxford have been discovered to contain honey.

American Chemical Society

The findings from the ancient Greek pot located at a shrine near Pompeii serve as evidence of the lasting nature of honey jars.

In 1954, a Greek burial shrine dating back to around 520 BC was uncovered in Pestum, Italy, approximately 70 kilometers south of Pompeii.

The shrine contained eight pots with sticky residues, and their contents remained a mystery since their unearthing.

Honey was initially suspected in tests conducted on one of the pots between the 1950s and 1980s by Luciana Carvalho from Oxford University.

Three distinct teams analyzed the residue but concluded that the jars contained animal or vegetable fats mixed with pollen and insect parts, rather than honey.

At that time, researchers depended on significantly less sensitive analytical methods, focusing on solubility tests.

Carvalho and her team started by examining the infrared reflection of the residues to determine their overall composition.

Ancient honey residues from the interior of the pot

Luciana da Costa Carvalho

Initially, it was hypothesized that the contents could be decomposed beeswax due to its outward resemblance and high acidity.

To test this hypothesis, the team employed gas chromatography paired with mass spectrometry, which ultimately unveiled the presence of sugars like glucose and fructose, the primary sugars found in honey.

“We unearthed a remarkably intricate mixture of acids and broken-down sugars,” states Carvalho. “The clear indicator of honey was the detection of sugar at the core of the residue.”

Further examination by Elizabeth Pierce from Oxford University confirmed the presence of a protein called major royal jelly protein, secreted by honeybees, along with the detection of peptide traces from Tropilaelaps Mercedesae, a parasitic mite that consumes bee larvae.

“This parasite is believed to derive from an Asian beehive,” Pierce comments.

Carvalho mentions that the cork seal of the bronze jar eventually failed, allowing air and microorganisms to enter. “We believe these bacteria consumed most of the sugar remnants, leading to the production of additional acids and decomposition products. What was left was an acidic, waxy residue clinging to the walls of the jar.”

“Investigating the honey offerings at the shrines in Paestum elucidates how the people honored their deities and their perceptions regarding the afterlife,” Carvalho explains.

Historic Herculaneum – discover Vesuvius, Pompeii, ancient Naples

The journey through history and archaeology embarks on a fascinating exploration where the past comes alive through Mount Vesuvius and the remains of Pompeii and Herculaneum.

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Source: www.newscientist.com

Australian Stingless Bee Honey Exhibits Unique Antibacterial Properties, Research Reveals

Honey sourced from Australian stingless bees, often referred to as sugar bag honeybees, has long been a vital food source and a traditional remedy for ailments such as itching and pain among Indigenous Australian communities. A recent study has examined the antibacterial properties and chemical composition of honey from three species of Australian stingless bees: Tetragonula carbonaria, Tetragonula hockingsii, and Austroplebeia australis. The results revealed these honeys possess strong antibacterial capabilities against various human pathogens, exhibiting efficacy on par with or superior to that of most European honeys derived from Apis mellifera.

Tetragonula carbonaria comb. Image credit: Tobias Smith.

Antibiotic resistance poses a significant global health issue.

Numerous natural substances have developed complex defenses against microbial threats and may serve as potential therapeutic agents.

While the medicinal qualities of European honey have been extensively researched, the therapeutic potential of Australian stingless bee honey is still in its early stages.

Kenya Fernandez, a researcher from the University of Sydney, noted:

“Unlike honey from European honeybees, which predominantly depends on hydrogen peroxide for its antibacterial effects, the honey from Australian stingless bees demonstrates high levels of hydrogen peroxide along with non-peroxide activity, making it a robust and versatile candidate for therapy.”

The study found that the honey retained antibacterial properties even without hydrogen peroxide, indicating that these traits are inherent to the honey.

“Bee Manuka honey’s notable non-oxide antibacterial activity is a key factor behind its commercial success,” Dr. Fernandez added.

“However, it heavily relies on a specific nectar source from the Myrtle plant (Leptospermum).”

“In contrast, the consistent antibacterial effectiveness of heat-treated non-toxic honey from Australian bees—regardless of various locations and nectar sources—highlights the unique qualities of these bees.”

Professor Dee Carter at the University of Sydney remarked:

Researchers are hopeful that this reliability will enhance the potential for commercial health applications.

Nonetheless, challenges concerning scalability persist. Each stingless bee hive produces roughly 0.5 liters of honey annually, complicating large-scale production.

“Although the yields are modest, these hives require less upkeep than traditional bee hives, enabling beekeepers to manage a greater number of hives,” stated Dr. Ross Groag from the University of Sydney.

“Encouragingly, the commercial value of honey may foster the growth of more hives and pave the way for scalability in commerce.”

“In the past year, native stingless bee honey has made strides toward local and global commercialization, having received endorsements from Australian and New Zealand food safety authorities.”

“This regulatory backing is crucial in establishing a niche market for high-value, low-volume products.”

The survey results were published on May 21st in the journal Applied and Environmental Microbiology.

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Kenya E. Fernandez et al. 2025. Potent antibacterial activity and unique physicochemical properties of honey from Australian stingless bees Tetragonula carbonaria, Tetragonula hockingsii, and Austroplebeia australis. Applied and Environmental Microbiology 91 (6); doi:10.1128/aem.02523-24

Source: www.sci.news

Honey, Sweetie, Dear: The Risks of ‘Elderspeak’

An illustrative instance of Elderspeak: Cindy Smith was spending time with her father in his assisted living apartment located in Roseville, California.

“He just shot her a look from beneath his bushy brows and asked, ‘What are we, married?'” she recounted.

Her father, 92 years old, was a former county planner and a World War II veteran. Although macular degeneration affected his eyesight and he navigated with caution, his cognitive faculties remained sharp.

“He usually isn’t very placid with others,” Smith noted. “But he felt he was an adult, and he often wasn’t treated as such.”

Most people intuitively grasp what “Elderspeak” entails. “What resembles baby talk is often directed toward the elderly,” stated Clarissa Shaw, a dementia care researcher and co-author affiliated with the University of Iowa College of Nursing. Recent Articles document its prevalence in research.

“It emerges from ageist assumptions of fragility, incapability, and reliance.”

This aspect may also involve inappropriate affection. “Elderspeak behaves like a superior, incorporating terms like ‘honey,’ ‘dearie,’ and ‘sweetie’ to dulcet the communication,” remarked Kristine Williams, a nurse gerontologist from the University of Kansas’s Faculty of Nursing and another co-author.

“We hold negative stereotypes about older individuals, prompting changes in our speech.”

Alternatively, caregivers might resort to using various pronouns. Are you ready for a bath? In this case, “they don’t act as individuals,” Dr. Williams explained. “I certainly hope I’m not bathing with you.”

Occasionally, Elderspeakers utilize loud, brief sentences or simple words delivered slowly. They may also employ an exaggerated singing tone more fitting for children, using terms like “toilet” or “jammies.”

With the so-called tag question – It’s lunchtime now, right? – “You’re posing questions but not allowing them to answer,” Dr. Williams clarified. “You’re telling them how to respond.”

Research in nursing homes highlights how prevalent such speech patterns are. This was evident when Dr. Williams, Dr. Shaw, and their team evaluated video recordings of 80 engagements between staff and dementia patients, finding that 84% involved some variant of Elderspeak.

“Most Elderspeak is well-meaning. People genuinely aim to assist,” Dr. Williams noted. “They fail to recognize the negative implications inherent in it.”

For instance, research among dementia patients in nursing homes has identified a correlation between exposure to Elderspeak and behaviors collectively referred to as resistance to care. Research indicates.

“Individuals might turn away, cry, or refuse,” Dr. Williams elucidated. “They could close their mouths during feeding attempts.” Some may even push caregivers away or become aggressive.

She and her team created a training initiative called Chat (Change Talk), a three-hour session that incorporates videos of communication between staff and patients, aiming to mitigate the use of Elderspeak.

The initiative proved effective. Prior to the training, encounters in 13 nursing homes located in Kansas and Missouri revealed that roughly 35% of staff interactions involved Elderspeak. This figure has now dropped to around 20%.

Simultaneously, resistance behaviors constituted nearly 36% of interaction time. Post-training, this percentage decreased to approximately 20%.

Additionally, a study carried out at Midwest Hospital found the same decline in resistance behaviors among dementia patients. The findings indicated.

Furthermore, the implementation of chat training in nursing homes was linked to a reduction in antipsychotic medication usage. While the results did not achieve statistical significance, they were deemed “clinically significant” by the researchers due to the small sample sizes involved.

“Many of these medications carry a black box warning from the FDA,” Dr. Williams mentioned. “Their use in frail elderly populations can be perilous due to potential side effects.”

Currently, Dr. Williams, Dr. Shaw, and their colleagues have streamlined the chat training for online implementation. They are assessing its effectiveness across around 200 nursing homes nationwide.

Even without a structured program, individuals and organizations can combat Elderspeak. Kathleen Carmody, the owner of Senior Matters Home Care and Consulting in Columbus, Ohio, suggests that when addressing clients, one should use titles like Mr. or Mrs., unless instructed otherwise.

However, in long-term care settings, families and residents may express concerns that altering staff communication could lead to resentment.

A few years ago, Carol Fahe dealt with a mother who was vision-impaired at an assisted living facility near Cleveland, becoming increasingly dependent in her 80s.

She described staff members who called her mother “sweetie” and “honey,” hovering over her while tying her hair in pigtails, likening the treatment to how toddlers are treated, said Fahe, 72, a psychologist from Kaneohe, Hawaii.

She recognized the aides meant well, but “there’s a misleading notion associated with that,” she reflected. “It doesn’t feel good for anyone. It’s isolating.”

Fahe contemplated addressing her concerns with the aide but hesitated, fearing retaliation. Ultimately, she moved her mother to a different facility for various reasons.

However, opposing Elderspeak doesn’t need to be confrontational, Dr. Shaw emphasized. Residents, patients, and individuals encountering Elderspeak elsewhere can respectfully express their preferences regarding how they wish to be addressed and what names they prefer, which is often applicable beyond healthcare environments.

Cultural variances also play a significant role. Felipe Agudero, a health communication educator at Boston University, pointed out that in specific contexts, endearing terms or phrases “aren’t rooted in underestimating someone’s intellect. They represent affection.”

Having moved from Colombia, he noted that his 80-year-old mother does not take offense when a physician or healthcare staff asks her to “Tómesela pastilita” (take this little pill) or “Muévanlas manitas” (move your little hands).

Such expressions are customary and “she feels as though she’s conversing with someone who cares,” Dr. Agudero conveyed.

“Arrive at a place of negotiation,” he advised. “There’s no need for confrontation. Patients have every right to state, ‘I prefer not to be spoken to in that manner.’ “

In response, professionals should “acknowledge that the recipient may not share the same cultural background,” he noted, adding, “This is how I communicate, but I can adapt.”

Lisa Graeme, 65, a retired writer from Alvada, Colorado, recently confronted Elderspeak when she enrolled in Medicare drug coverage.

She recalled receiving nearly daily calls from mail-order pharmacies, following their failure to meet her prescription needs.

These “overly sweet” callers seemed to follow a script, addressing her as “Mr. Graeme,” as if they were administering medication.

Frustrated by their assumptions and their probing questions about her medication adherence, Ms. Graeme informed them that she had sufficient stock, thanks. She organizes her own refills.

“I asked them to cease calling,” she recounted. “And they did.”

The New Old Age, KFF Health News.

Source: www.nytimes.com

Harnessing Clues from Honey to Address Biodiversity’s Greatest Challenges

Foraging bees carry pollen from surrounding plants back to the hive

Eric Tourneret

If you’re lucky enough to find one, dip your fingers into a jar of honey from Pitcairn Island, a remote British Overseas Territory in the South Pacific. Honey is the island’s main export and highly prized among foodies. Pure and free of pesticides and pollutants, the honey infuses the delicious subtropical scents of Pitcairn Island plants, including passionflower, mango, guava and rose apple.

Honey contains much more information, if only we knew how to look for it. Breaking a container of honey opens a gateway to an entire ecosystem. Honey contains a detailed record of everything the bee encountered while foraging – not just the flowers that provided it with nectar and pollen, but also other plants, insects, fungi, viruses and larger animals in the environment.

He explains that honeybees are “passive bioaccumulators.” Parwinder Kaur As the bees go about their day, their fluffy bodies unwittingly collect samples of whatever they come into contact with, which then mix into the honey the bees produce, says researcher David Schneider of the University of Western Australia in Perth. “It’s got everything in it,” he says. Luca Fontanesi at the University of Bologna in Italy. “Well, almost everything.”

Scientists are now realizing that they have the potential to reach into that honey pot and garner sweet morsels of information that are currently hard to come by. Using advanced DNA tools, researchers are using honey to gather data on bee health, the general state of biodiversity in the bees’ foraging areas, and disease in the broader environment. They could identify possible culprits behind the mysterious Colony Collapse Disorder that is wiping out beehives, and…

Source: www.newscientist.com