Doctor Seeks Individuals with Exceptional Stool Quality for Health Study

Infectious disease expert Elizabeth Homan with stool donor Dmitri

Elizabeth Homan with her valuable stool donor, Mr. Dmitri

Elizabeth Homan

Fecal transplants have become a vital treatment for Clostridioides difficile relapses. However, sourcing high-quality stool donors remains a significant challenge.

“This process can be quite frustrating; only about 1 percent of those who respond to donor ads are in optimal health,” states Elizabeth Homan, an infectious disease specialist at Massachusetts General Hospital in Boston. “Finding qualified donors is tough, so we really appreciate their generosity.” Over the years, some donors have contributed their stools over 100 times.

Homan has overseen the fecal transplant program at her hospital for 15 years. Her responsibilities include collecting donations, processing them into oral capsules, and administering them to patients suffering from challenging intestinal conditions, particularly recurrent C. difficile infections that are resistant to antibiotics. The beneficial gut bacteria in the donor’s stool help to eliminate harmful bacteria in the recipient’s gut, alleviating symptoms.

To recruit donors, Homan advertises online, offering $1,200 for a month’s worth of stool donations.

Potential donors undergo a rigorous screening process. Many do not advance past the initial phone interview due to health criteria, such as being a healthcare worker or recent travel to Southeast Asia, both of which heighten the risk of transmitting drug-resistant bacteria. Furthermore, donors need to be within a healthy weight range, as past experiences showed that stools from obese donors could cause adverse reactions in recipients.

Individuals who pass the initial screening undergo comprehensive testing, including blood tests to evaluate their overall health, screenings for infectious diseases like HIV and COVID-19, and rectal examinations to detect any intestinal abnormalities.

Homan’s most successful donors are often fitness enthusiasts with balanced diets. One notable donor is a “semi-professional athlete, personal trainer, and gym owner.” Generally, superior stools result from diets rich in natural foods while minimizing ultra-processed options. “We’ve considered using only vegan donors, but in reality, my best donors have been omnivores,” she notes.

Donation periods typically last from 2 to 4 weeks. During this time, donors are encouraged to make frequent visits to the hospital for donations. “They often have regular bowel movements, coming in around the same time daily after a coffee boost,” Homan explains. Each stool sample is collected in a plastic container and processed in the lab.

Fresh stool is quickly converted into capsules. “I blend it with saline and strain it through a graduated mesh filter,” Homan explains. After additional processing, the liquid is encapsulated. “It’s not pleasant, but you adapt,” she adds.

After each donation period, donors are screened again for any infections, making sure they are not exposed to pathogens like Salmonella. If they test positive, the capsules are discarded, and new donor sourcing begins.

Despite these hurdles, Homan expresses her passion for the job, highlighting the life-changing effects fecal transplants can have on patients. Recently, a patient who was unable to work has returned to a 30-hour work week thanks to the transplant capsules. “I continue this work because it makes a meaningful difference in people’s lives,” she remarks.

Sadly, Elizabeth Homan is nearing retirement and is struggling to find a replacement. “I keep asking my department, ‘Who’s willing to help?’ The response has been silence. It seems they’re overwhelmed with the basics and hesitant to take on this responsibility.”

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Source: www.newscientist.com

Simplified diagnosis of endometriosis possible with stool test

Endometriosis tissue seen under a microscope

Bio Photo Associates/Science Photo Library

Low levels of certain compounds in your feces may be a sign of endometriosis, and supplementing with those compounds may also help control your symptoms.

Endometriosis, which affects about 200 million people worldwide, occurs when the tissue lining the uterus grows in other parts of the reproductive tract. There is no known cure, but once the condition is diagnosed, lesions can be removed periodically through surgery. However, it currently takes more than six years on average for endometriosis to be diagnosed, largely due to a lack of awareness and understanding.

Previous research suggests that the gut microbiome may play a role in this condition. To investigate further, ramakrishna konmagani and colleagues at Baylor College of Medicine in Houston, Texas, collected stool samples from 18 women with endometriosis and 31 women without endometriosis. They investigated the bacteria in feces and the metabolome, the set of chemicals produced by gut bacteria.

They found that women with endometriosis had lower levels of the metabolite 4-hydroxyindole in their feces. This is likely due to changes in the gut microbiome.

Based on this finding, commercially available stool analysis could enable rapid screening of this widely “underdiagnosed, understudied, and underdiagnosed” condition, potentially leading to early and effective management. Konmagani said.

“Stool sampling is very easy and not as invasive as current diagnostic techniques such as laparoscopy.” [a kind of keyhole surgery]” he says.

To test whether 4-hydroxyindole had a protective effect, the research team gave additional 4-hydroxyindole to a group of mice that had tissue implanted in their abdomens to induce endometriosis. After 14 days of treatment, these mice did not have fewer lesions compared to control animals, but the lesions were significantly less severe and showed signs of significantly reduced pain.

Further experiments showed that administering 4-hydroxyindole to mice with established endometriosis significantly improved the lesions. The results were similar in mice transplanted with human endometriosis lesions, suggesting that the treatment may be equally effective in humans.

“We believe this is a very good treatment option because it is something that occurs naturally in the body and is not drugged or synthetic,” Konmaghani said. I say.

However, large-scale human studies will be needed to confirm whether 4-hydroxyindole can be used to diagnose endometriosis and whether the compound is effective as a treatment.

topic:

  • women's health/
  • microbiome

Source: www.newscientist.com

Why Autism could soon be identifiable through stool samples – the breakthrough explained

New Research has introduced an innovative and cost-effective method for diagnosing autism, potentially simplifying and speeding up the diagnostic process. Surprisingly, the new diagnostic test might involve analyzing stool samples rather than brain scans or psychiatric evaluations. Researchers have discovered consistent differences in the gut microbiome – the population of microorganisms in the intestine – between individuals with autism and those without.

Regularly collecting and analyzing stool samples could potentially enable doctors to identify autism at an earlier stage than current methods allow. This new approach is especially important given the diverse range of symptoms associated with autism, making diagnosis challenging.


“There is a pressing need to delay the diagnosis of autism in children, as the symptoms can vary widely,” according to the co-authors of the study. Professor Shu Qian Ng told BBC Science Focus.

Autism is a common condition, affecting approximately 1 in 100 individuals worldwide. While genetics is thought to play a significant role in autism, other factors such as birth complications, exposure to air pollution, and gender differences may also contribute.

Diagnosing autism currently relies on subjective behavioral assessments and developmental screenings, which can be time-consuming and depend on the availability of trained professionals. The average age of diagnosis in the United States is around 5 years old, resulting in delays in essential care and support for children with autism during crucial developmental stages.

The study, published in Nature Microbiology, analyzed stool samples from 1,627 children between 1 and 13 years of age, some of whom had autism. The researchers identified significant differences in gut bacteria between children with autism and those without, resulting in the identification of specific biomarkers for autism.

Utilizing machine learning algorithms, scientists could determine autism in children with up to 82% accuracy based on the biological function of several microorganisms in the digestive system. This innovative approach offers hope for a non-invasive and efficient diagnostic test for autism, expected to be available by the end of the year.

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How is the gut microbiome linked to autism?

Dr. Ng explains two primary differences in the gut microbiome of individuals with autism compared to neurotypical individuals. Firstly, individuals with autism tend to have reduced diversity in their gut microbiome, with fewer types of bacteria and viruses. Secondly, children with autism often have an abundance of pathogens in their gut, potentially contributing to their condition.

The ongoing research aims to determine whether interventions such as dietary modifications or probiotics can help improve gut diversity and health, potentially influencing autism symptoms. Early indications suggest that adjusting the microbiome can lead to symptom improvements in children with autism.

While promising, the research does not establish causation between gut biomarkers and autism. Clinical trials are underway to explore the potential of using stool samples as a diagnostic tool for autism in young children.

The study participants were predominantly Chinese, highlighting the need for further global studies to enhance diagnostic accuracy across diverse populations. Despite potential dietary influences, preliminary findings suggest consistent diagnostic performance regardless of dietary habits.

About our experts

Dr. Shu N, a Professor and Associate Dean at The Chinese University of Hong Kong, specializes in investigating the potential of gut microbiota for diagnosing and treating various diseases, including autism. Her research focuses on using microbial markers to predict disease risks and develop personalized interventions for improving health outcomes.

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Source: www.sciencefocus.com